Characterization of the physicochemical properties of the biosurfactant produced by L. acidophilus and L. pentosus
Keywords:
Biosurfactant, Emulsification index, Surface tension, critical micelle concentration, L. acidophilus, L. pentosus
Abstract
Biosurfactants or surface-active compounds are biodegradable, non-toxic and ecofriendly
compounds released by microorganisms. Biosurfactants are amphiphilic compounds cause
surface tension reduction both aqueous solutions and hydrocarbon mixtures. The main purpose
of this work was to characterize biosurfactant produced by Lactobacillus strains. Identification
using 16s rDNA identified the isolates as L. acidophilus for Fm1 and L. pentosus for Y1. Effect
different parameters (temperature, PH and Salinity) were studied to evaluate the stability of
biosurfactant after treatment. In addition, critical micelle concentration of biosurfactant,
emulsification index and viscosity reduction of palm and engine oils have been studied. The
results revealed that, the biosurfactant from L. acidophilus and L. pentosus maintains its
emulsifications activities unaffected in the wide range of parameter's study except slightly
decreasing in emulsification index values at salinity 15%. The maximum reduction in surface
tension was 18.05 mN/m with minimum concentration of critical micelle concentration of 7.5
mg/ml and high decrease of palm and engine oil viscosity of 110.1 and 165.3% respectively. This
study concluded that, the emulsification activity, the surface activity and the stability to heat
treatment, different PH and salinity of biosurfactant of Lactobacillus strains revealed the
application of the biosurfactant in food, pharmaceutical, cosmetics industries and oil recovery.
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Microbiol Biotechnol 2:827–837.
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biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresource
Technology. Vol. 97: p. 868-875.
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Bacterial Pathogens. Environment Asia. Vol. 4 (1): p. 1-5.
26 Bento, M., Camargo, F. D. O., Okeke, BC & Frankenberger WT Jr. 2005. Diversity of biosurfactant
producing microorganisms isolated from soils contaminated with diesel oil. Microbiol Res. Vol.
160 (3): p. 249-255.
27 Satpute, S.K., Banat, I.M., Dhakephalkar, P.K., Banpurkar, A.G., et al., 2010. Biosurfactants,
bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol. Adv., 28, 436–
450.
28 Cooper, D. G., C. R. MacDonald, S. J. B. Duff, & N. Kosaric. 1981. Enhanced production of
surfactin from Bacillus slubtilis by continuous product removal and metal cation additions. Appl.Environ. Microbiol., 42:408-412, 1981.
29 Nadielly R. Andrade Silva, Marcos A. C. Luna, André L. C. M. A. Santiago, Luciana O. Franco,
Grayce K. B. Silva, Patrícia M. de Souza, Kaoru Okada, Clarissa D. C. Albuquerque, Carlos A. Alves
da Silva, andGalba M. Campos-Takaki. 2014. Biosurfactant-and-Bioemulsifier Produced by a
Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil.
Campos-Takaki Int. J. Mol. Sci. 2014, 15, 15377-15395.
30 Sen, R. & Swaminathan, T. 2005. “Characterization of concentration and purification parameters
and operating conditions for the small-scale recovery of surfactin, Process”. Biochem. Vol. 40: p.
2953–2958.
advantages to microorganisms and mankind. Adv. Exp. Med. Biol., 672:261–280.
2. Mukherjee, S., Das, P., & Sen, R. 2006. Towards commercial production of microbial
surfactants.Trends Biotechnol 24, 509–515.
3. Banat. I. M., Franzetti.A., Gandolfi. I., Bestetti . G,m Martinotti M. G., Fracchia, L., Smyth, T.J., &
Marchant, R. 2010. Applied Microbiology and Biotechnology June, 87, 2: 427 444.
4. Mcinerney, M. J., Javaheri, M. & Nagle, D. P. 1990. Properties of the biosurfactant produced by
Bacillus licheniformis strain JF-2. J. Ind. Microbiol., 5, 95-102.
5. Conter, M., Muscariello, T., Zanardi, E., Ghidini, S., Vergara, A., Campanini, G. & Lanieri, A. 2005.
“Characterization of Lactic Acid Bacteria Isolated from an Italian Dry Fermented Sausage”.
Romanian Biotechnological Letters. Vol. 14: p. 167-174.
6. Yin, H., J. Qiang, Y. Jia, J. Ye, Peng, H. Qin, N. Zhang & B. He. 2009. Characteristics of
biosurfactant produced by Pseudomonas aeruginosa S6 isolated from oil-containing wastewater.
Process Biochemistry. Vol. 44: p. 302-308.
7. Cooper, D. G & Goldenberg, B. G. 1987. Surface-Active Agents from Two Bacillus Species. Appl
Environ Microbiol , 53(2): p. 224–229.
8. El-Sersy, N. 2012. Plackett-Burman design to optimize biosurfactant production by marine
Bacillus subtilis N10. Rom. Biotechnol. Lett. 17 (2), 7049–7064.
9. Anandaraj B, Thivakaran P. 2010. Isolation and production of biosurfactant producing organism
from oil spilled soil. J Biosci Tech 1: 120–126.
10. Devesa-Rey R., Bustos G., Cruz, J.M. & Moldes, A.B. 2011. Optimisation of entrapped activated
carbon conditions to remove coloured compounds from winery wastewaters. Bioresource
Technology, 102, 6437–6442.
11. Jara AM, Andrade RF, & Campos-Takaki GM. 2013. Physicochemical characterization of tensioactive produced by Geobacillus stearothermophilus isolated from petroleum-contaminated soil.
Colloids Surf. B Biointerf. 101:315-318.
12. Augustin, M & Hippolyte, M. T. 2012. Screening of biosurfactants properties of cell-freesupernatants of cultures of Lactobacillus spp. isolated from a local fermented milk (Pendidam) of
Ngaoundere (Cameroon). International Journal of Engineering Research and Applications. Vol. 2
(5): p. 974-985.
13. Desai, J.D., & Banat, I.M. 1997. Microbial production of surfactant and their commercial
potential. Microbiology and Molecular Biology Reviews. Vol. 61: p.47–64, 1997.
14. Eduardo J., Gudina, J., José A., Teixeira & Lígia R. Rodrigues. 2011. Biosurfactant-Producing
Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition. Applied and
Environmental Soil Science. Article ID 201254.
15. Khopade, A., Biao, R., Liu, X., Mahadik, K., Zhang, L & Kokare, C. 2012. Production and stability
studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination 3, 198–204.
16. Augustin, M., Hippolyte, M.T & Ra€ıssa, K.R., 2013. Antibacterial activity of Lactobacillus’
biosurfactants against Pseudomonas spp. isolated from fresh beef. Novus Int. J. Biotechnol.
Biosci., 2, 7–22.
17. Gudina, E. J., Teixeira, J.A. & Rodrigues, L. R. (2010). Isolation and functional characterization of
a biosurfactant produced by Lactobacillus paracasei. Colloids and Surfaces B: Biointerfaces,
76:298-304.
18. Augustin, M., Majesté, P. M., Hippolyte, M.T & Léopold, T. N. 2015. Effect of Biosurfactants
Extracted from a Locally Fermented Milk (Pendidam) on Its Shelf Life. Journal of Advances in
Biology & Biotechnology. Vol. 3(1): p. 12-22.
19. Rodrigues, L., Banat, IM., Teixeira, J & Oliveira, R. 2006. Biosurfactants: potential applications in
medicine. J Antimicrob Chemother. Vol. 57: p. 609–618.
20. Joshi, S., Bharucha, C. & Anjana. 2008. “Production of biosurfactant and antifungal compound by
fermented food isolate Bacillus subtilis 20B”. J. Desai Bioresource Technology. Vol. 99. p. 4603–
4608.
21 Fernandes, E.C.R et al. 2013. “Study of Biosurfactant “Cocktails” with Enhanced Properties.
(Master Thesis)”. Minho's University, Portuguese. pp. 3.
22 Rodrigues, L., Teixeira, J., Oliveira, R. & Van der Mei, H. 2005. “Response surface optimization of
the medium components for the production of biosurfactants by probiotic bacteria”. Process
Biochemistry. Vol. 41: p.1-10.
23 Fracchia L, Cavallo M, Allegrone G & Martinotti MG. 2010. A Lactobacillus-derived biosurfactant
inhibits biofilm formation of human pathogenic Candida albicans biofilm producers. Appl
Microbiol Biotechnol 2:827–837.
24 Batista, S.B., A.H. Mounteer, F.R. Amorim, & M.R. Totola. 2006. Isolation and characterization of
biosurfactant/bioemulsifier-producing bacteria from petroleum contaminated sites. Bioresource
Technology. Vol. 97: p. 868-875.
25 Siripun, S. 2011. Isolation of Biosurfactant–Producing Bacteria with Antimicrobial Activity against
Bacterial Pathogens. Environment Asia. Vol. 4 (1): p. 1-5.
26 Bento, M., Camargo, F. D. O., Okeke, BC & Frankenberger WT Jr. 2005. Diversity of biosurfactant
producing microorganisms isolated from soils contaminated with diesel oil. Microbiol Res. Vol.
160 (3): p. 249-255.
27 Satpute, S.K., Banat, I.M., Dhakephalkar, P.K., Banpurkar, A.G., et al., 2010. Biosurfactants,
bioemulsifiers and exopolysaccharides from marine microorganisms. Biotechnol. Adv., 28, 436–
450.
28 Cooper, D. G., C. R. MacDonald, S. J. B. Duff, & N. Kosaric. 1981. Enhanced production of
surfactin from Bacillus slubtilis by continuous product removal and metal cation additions. Appl.Environ. Microbiol., 42:408-412, 1981.
29 Nadielly R. Andrade Silva, Marcos A. C. Luna, André L. C. M. A. Santiago, Luciana O. Franco,
Grayce K. B. Silva, Patrícia M. de Souza, Kaoru Okada, Clarissa D. C. Albuquerque, Carlos A. Alves
da Silva, andGalba M. Campos-Takaki. 2014. Biosurfactant-and-Bioemulsifier Produced by a
Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil.
Campos-Takaki Int. J. Mol. Sci. 2014, 15, 15377-15395.
30 Sen, R. & Swaminathan, T. 2005. “Characterization of concentration and purification parameters
and operating conditions for the small-scale recovery of surfactin, Process”. Biochem. Vol. 40: p.
2953–2958.
Published
2018-11-30
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